Published customer image: LYNX Rapid Plus Biotin (Type 1) Antibody Conjugation Kit (LNK262B) used to biotinylate human myelin basic protein prior to in vitro MBP binding assays wirh human B cells assessed by flow cytometry.Image caption:Serum complement promotes the binding of MBP to normal B cells.PBMCs from healthy donors were incubated for 30 min with or without 30 μg/ml biotinylated MBP in medium containing normal serum (30% v/v), or in pure medium. (A) Histogram plot depicting MFI values of MBP binding to B cells in one representative healthy donor. B) The binding of MBP to B cells from 7 healthy donors is shown, expressed as percentage MBP-positive B cells. C) Before addition to the culture media, serum was treated in one of three ways: heat-inactivated (h.i.) by heating to 56°C for 30 min, or supplemented with EDTA or sodium polyanethole sulphonate (SPS) in different concentrations. The resulting MFI values from 5–7 healthy donors are shown. Bars and error bars represent means and SEM *p<0.05 **p<0.01 and ***p<0.001.From: Brimnes MK, Hansen BE, Nielsen LK, Dziegiel MH, Nielsen CH (2014)Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells.PLoS ONE 9(11): e113388.doi: 10.1371/journal.pone.0113388This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Published customer image: LYNX Rapid Plus Biotin (Type 1) Antibody Conjugation Kit (LNK262B) used to biotinylate human myelin basic protein prior to in vitro MBP binding assays wirh human B cells assessed by flow cytometry.Image caption:Serum complement promotes the binding of MBP to normal B cells.PBMCs from healthy donors were incubated for 30 min with or without 30 μg/ml biotinylated MBP in medium containing normal serum (30% v/v), or in pure medium. (A) Histogram plot depicting MFI values of MBP binding to B cells in one representative healthy donor. B) The binding of MBP to B cells from 7 healthy donors is shown, expressed as percentage MBP-positive B cells. C) Before addition to the culture media, serum was treated in one of three ways: heat-inactivated (h.i.) by heating to 56°C for 30 min, or supplemented with EDTA or sodium polyanethole sulphonate (SPS) in different concentrations. The resulting MFI values from 5–7 healthy donors are shown. Bars and error bars represent means and SEM *p<0.05 **p<0.01 and ***p<0.001.From: Brimnes MK, Hansen BE, Nielsen LK, Dziegiel MH, Nielsen CH (2014)Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells..doi: 10.1371/journal.pone.0113388This image is from an open access article distributed under terms of a.
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